The Microbiome Facility provides the following services:
- Biosample banking
- Automated DNA/RNA extraction
- Amplicon-sequencing (16S, ITS) (Miseq, 2X300 cycles run)
- Shotgun metagenomics (Nextseq, 2X150 cycles; Novaseq, 2X250 cycles)
- Advanced ‘omics (Metatranscriptomics)
- Microbiome analysis from animal models
- Bioinformatics support
- Droplet digital PCR (ddPCR) for ultrasensitive quantification of the total bacterial load and the target species
- mIHC to evaluate intra-tumoral microbiome
- FMT and gnotobiotic mice model establishment
The Microbiome Facility utilizes the following instrumentation:
- Anaerobic hood (FMT lab)
- BEAD BlasterTM 24 homogenizer
- QIAcube DNA/RNA extraction automation
- Centrifuge
- NanoDrop 2000
- PCR workstation
- Qubit™ 4 fluorometer (DNA library quantification)
- Agilent 2100 bioanalyzer system (DNA library QC)
- Fridge and freezers
- Miseq
- Nextseq550 (CGF)
- Novaseq (UGC)
- QX200 droplet digital PCR system (Biorad)
- PhenoImager™ HT (formerly Vectra ® Polaris™) (Akoya Biosciences)
Fecal Microbiota Transplant Lab
Bio-banking and Genomics Lab
