Services and Instrumentation

 

The Microbiome Facility provides the following services:

  • Biosample banking
  • Automated DNA/RNA extraction
  • Amplicon-sequencing (16S, ITS) (Miseq, 2X300 cycles run)
  • Shotgun metagenomics (Nextseq, 2X150 cycles; Novaseq, 2X250 cycles)
  • Advanced ‘omics (Metatranscriptomics)
  • Microbiome analysis from animal models
  • Bioinformatics support
  • Droplet digital PCR (ddPCR) for ultrasensitive quantification of the total bacterial load and the target species
  • mIHC to evaluate intra-tumoral microbiome
  • FMT and gnotobiotic mice model establishment

The Microbiome Facility utilizes the following instrumentation:

  • Anaerobic hood (FMT lab)
  • BEAD BlasterTM 24 homogenizer
  • QIAcube DNA/RNA extraction automation
  • Centrifuge
  • NanoDrop 2000
  • PCR workstation
  • Qubit™ 4 fluorometer (DNA library quantification)
  • Agilent 2100 bioanalyzer system (DNA library QC)
  • Fridge and freezers
  • Miseq
  • Nextseq550 (CGF)
  • Novaseq (UGC)
  • QX200 droplet digital PCR system (Biorad)
  • PhenoImager™ HT (formerly Vectra ® Polaris™) (Akoya Biosciences)
Fecal Microbiota Transplant Lab

Fecal Microbiota Transplant Lab

Bio-banking and Genomics Lab

Bio-banking and Genomics Lab