MWRI Work-in-Progress Conference and Seminar Series

April 21, 2021 @ 12:00 pm – 1:00 pm
Dr. Mellissa Mann
Speaker: Kai Ding
Title: Graduate Student, University of Pittsburgh School of Medicine
Lecture Title: “In Depth Single Cell Profiling of a Case of Bone Metastases With Associated Organoid Models Reveals a Precision Medicine Approach to Treatment”

Please join us, VIRTUALLY, for our MWRI/WIP Series, Wednesday, April 21, 2021 @ 12pm




Work-in-Progress Conference & Seminar Series


“In depth single cell profiling of a case of bone metastases with associated organoid models reveals a precision medicine approach to treatment”

Kai Ding, Graduate Student,

University of Pittsburgh School of Medicine – Pittsburgh, PA



  1. Occurring in 65-80% of metastatic breast cancer (BC), bone metastasis (BoM) is the major cause of BC related mortality. Like other cancers, BC BoM has high inter- and intra-patient heterogeneity. Thus, understanding disease evolution and heterogeneity of BoM at the individual patient level will be key to guide precise application of targeted therapies. In this study, we described in depth histologic and single cell molecular characterization of a case of invasive lobular breast cancer (ILC) metastasis to bone, and examined potential therapeutic targets with associated organoid models.




“Zinc protection of fertilized eggs is an ancient feature of sexual reproduction in animals”

Rachel Bainbridge, PhD Student,

Carlson Lab, University of Pittsburgh, Department of Biological Sciences

Molecular, Cell, and Developmental Biology Program




  1. In most sexually reproducing organisms, it is vital that the egg is fertilized by only one sperm. In order to ensure this occurs, eggs utilize polyspermy blocks, mechanisms that inhibit the entry of multiple sperm. Recently, it has been observed that mammalian eggs release zinc at activation, a phenomenon hypothesized to contribute to the polyspermy block. We seek to demonstrate that this zinc release is conserved in non-mammalian eggs, and to directly test zinc’s ability to inhibit fertilization.



Date: Wednesday, April 21, 2021
12:00 to 1:00 PM
Location: VIRTUALLYMagee-Women’s Research Institute, Conference Center, 1st Floor


For program questions, please contact Dr. Mellissa Mann @ 412-641-7950. 

CME and CEU credits can be earned by attending WIP and providing required information.