Cytometry Facility Protocols

Protocols

• AnnexinV/PI for apoptosis
• Cell Cycle Analysis
  • Cell cycle plus surface staining
  • Cell cycle plus checkpoint staining
  • Cell cycle plus proliferation with EDU
• CFSE staining for T-cells
• Compensation beads
• Ficol hypaque
• Fluorochrome titration
• Mitochrondria membrane potential with JC-1
• Red blood cell lysis
• Surface staining
  • Intracellular staining
  • Phospho-staining

References

• Donnenberg VS, Zimmerlin L, A.D. D. Role of ABC Transporters in Cancer Stem Cell Drug Resistance. In: Allan AL, editor. Cancer Stem Cells in Solid Tumors. New York: Springer Science and Business Media; 2011.
• Donnenberg VS, Donnenberg AD. Flow Cytometry on Disaggregated Solid Tissues. International Drug Discovery. 2011;August/September:1-3.
• Donnenberg VS, Meyer EM, Donnenberg AD. Measurement of Multiple Drug Resistance Transporter Activity in Putative Cancer Stem/Progenitor Cells. In: Yu J, editor. Methods in Molecular Biology. New York: Humana Press, Springer; 2009. p. 261-79.
• Donnenberg AD, Donnenberg VS. Understanding Clinical Flow Cytometry. In: O’Gorman MR, Donnenberg AD, editors. Handbook of Human Immunology. 2nd ed. Boca Raton: CRC Press Taylor and Francis; 2008. p. 181-220.
• Donnenberg AD. Statistics of Immunological Testing. In: O’Gorman MR, Donnenberg AD, editors. Handbook of Human Immunology. 2nd ed. Boca Raton: CRC Press Taylor and Francis; 2008. p. 29-62.
• Donnenberg AD, Donnenberg VS. Rare–event analysis in flow cytometry. Clin Lab Med. 2007;27(3):627-52, viii.
• Donnenberg AD, Donnenberg VS. Phenotypic and functional measurements on circulating immune cells and their subsets. In: Lotze MT, Thomson AW, editors. Measuring immunity : basic biology and clinical assessment. San Diego, Calif. ; London: Elsevier Academic Press; 2005. p. 237-56.