Protocols
- AnnexinV/PI for apoptosis
- Cell Cycle Analysis
- CFSE staining for T-cells
- Compensation beads
- Ficol hypaque
- Fluorochrome titration
- Mitochrondria membrane potential with JC-1
- Red blood cell lysis
- Surface staining
References
- Donnenberg VS, Zimmerlin L, A.D. D. Role of ABC Transporters in Cancer Stem Cell Drug Resistance. In: Allan AL, editor. Cancer Stem Cells in Solid Tumors. New York: Springer Science and Business Media; 2011.
- Donnenberg VS, Donnenberg AD. Flow Cytometry on Disaggregated Solid Tissues. International Drug Discovery. 2011;August/September:1-3.
- Donnenberg VS, Meyer EM, Donnenberg AD. Measurement of Multiple Drug Resistance Transporter Activity in Putative Cancer Stem/Progenitor Cells. In: Yu J, editor. Methods in Molecular Biology. New York: Humana Press, Springer; 2009. p. 261-79.
- Donnenberg AD, Donnenberg VS. Understanding Clinical Flow Cytometry. In: O’Gorman MR, Donnenberg AD, editors. Handbook of Human Immunology. 2nd ed. Boca Raton: CRC Press Taylor and Francis; 2008. p. 181-220.
- Donnenberg AD. Statistics of Immunological Testing. In: O’Gorman MR, Donnenberg AD, editors. Handbook of Human Immunology. 2nd ed. Boca Raton: CRC Press Taylor and Francis; 2008. p. 29-62.
- Donnenberg AD, Donnenberg VS. Rare–event analysis in flow cytometry. Clin Lab Med. 2007;27(3):627-52, viii.
- Donnenberg AD, Donnenberg VS. Phenotypic and functional measurements on circulating immune cells and their subsets. In: Lotze MT, Thomson AW, editors. Measuring immunity : basic biology and clinical assessment. San Diego, Calif. ; London: Elsevier Academic Press; 2005. p. 237-56.
Useful Links
General Cytometry
ISAC – International Society of Analytical Cytology
Purdue Cytometry Resource
GLIIFCA – Great Lakes International Imaging and Flow Cytometry Assoc.
Clinical Cytometry Society
National Flow Cytometry Resource
Pitt Labs
Flow Cytometry Facility – Dept of Immunology
Flow Cytometry Facility – Starzl Transplantation Institute
Center for Biological Imaging
Clinical Flow Cytometry Laboratory – Dept of Pathology
Spectral Viewers
Curve–O–Matic 2.0
Cytek Aurora Spectral Viewer
Fluorescence SpectraViewer
Cross-Reactivity Information
NIH Nonhuman Primate Reagent Resource
The Antibody Resource Page
Other Labs
University of Chicago
Roswell Park Cancer Institute
University of North Carolina
MD Anderson Cancer Center
Yale University
The Wistar Institute
Vendors
Amnis
Applied Cytometry
Beckman Coulter
Becton Dickinson
Cytonome
DAKO
eBioscience
Molecular Probes–Invitrogen
