Additional Information

Cytometry Facility Protocols

Protocols

• AnnexinV/PI for apoptosis (PDF)
• Cell Cycle Analysis (PDF)
  • Cell cycle plus surface staining (PDF)
  • Cell cycle plus checkpoint staining (PDF)
  • Cell cycle plus proliferation with EDU (PDF)
• CFSE staining for T-cells (PDF)
• Compensation beads (PDF)
• Ficol hypaque (PDF)
• Fluorochrome titration (PDF)
• Mitochrondria membrane potential with JC-1 (PDF)
• Red blood cell lysis (PDF)
• Surface staining (PDF)
  • Intracellular staining (PDF)
  • Phospho-staining (PDF)

References

• Donnenberg VS, Zimmerlin L, A.D. D. Role of ABC Transporters in Cancer Stem Cell Drug Resistance. In: Allan AL, editor. Cancer Stem Cells in Solid Tumors. New York: Springer Science and Business Media; 2011. (PDF)
• Donnenberg VS, Donnenberg AD. Flow Cytometry on Disaggregated Solid Tissues. International Drug Discovery. 2011;August/September:1-3. (PDF)
• Donnenberg VS, Meyer EM, Donnenberg AD. Measurement of Multiple Drug Resistance Transporter Activity in Putative Cancer Stem/Progenitor Cells. In: Yu J, editor. Methods in Molecular Biology. New York: Humana Press, Springer; 2009. p. 261-79.
• Donnenberg AD, Donnenberg VS. Understanding Clinical Flow Cytometry. In: O’Gorman MR, Donnenberg AD, editors. Handbook of Human Immunology. 2nd ed. Boca Raton: CRC Press Taylor and Francis; 2008. p. 181-220. (PDF)
• Donnenberg AD. Statistics of Immunological Testing. In: O’Gorman MR, Donnenberg AD, editors. Handbook of Human Immunology. 2nd ed. Boca Raton: CRC Press Taylor and Francis; 2008. p. 29-62. (PDF)
• Donnenberg AD, Donnenberg VS. Rare–event analysis in flow cytometry. Clin Lab Med. 2007;27(3):627-52, viii.
• Donnenberg AD, Donnenberg VS. Phenotypic and functional measurements on circulating immune cells and their subsets. In: Lotze MT, Thomson AW, editors. Measuring immunity : basic biology and clinical assessment. San Diego, Calif. ; London: Elsevier Academic Press; 2005. p. 237-56. (PDF)

Additional Links

General Cytometry

• ISAC – International Society of Analytical Cytology
• Purdue Cytometry Resource
• GLIIFCA – Great Lakes International Imaging and Flow Cytometry Assoc.
• Clinical Cytometry Society
• National Flow Cytometry Resource

Pitt Labs

• Flow Cytometry Facility – Dept of Immunology
• Flow Cytometry Facility – Starzl Transplantation Institute
• Center for Biological Imaging
• Clinical Flow Cytometry Laboratory – Dept of Pathology

Spectral Viewers

• Curve–O–Matic 2.0
• Cytek Aurora Spectral Viewer
• Fluorescence SpectraViewer

Cross-Reactivity Information

• NIH Nonhuman Primate Reagent Resource
• The Antibody Resource Page

Other Labs

• University of North Carolina
• MD Anderson Cancer Center
• Yale University
• The Wistar Institute

Vendors

• Amnis
• Applied Cytometry
• Beckman Coulter
• Becton Dickinson
• Cytonome
• DAKO
• eBioscience
• Molecular Probes–Invitrogen