Services provided by the Translational Pathology Imaging Laboratory include:
- Panel design and optimization (6 antibodies + DAPI)
- Automated multiplex staining on the Leica BOND RX
- Slide Imaging on the PhenoImager HT
- Region of interest selection
- Image analysis with inForm® – includes tissue/cell segmentation algorithms and quantification of individual biomarkers and phenotypes
- Additional custom analyses – biomarker co-expression, spatial relationships between cellular phenotypes
- inForm® workstation availability for independent use
Access the TPIL Intake Form. Email Maron Joy, PhD (joym@upmc.edu), with completed intake form to schedule initial project meeting.
Multiplex Staining and Imaging Workflow
![Sequential staining of seven markers on the BOND RX.](https://hillmanresearch.upmc.edu/wp-content/uploads/staining-on-BOND-RX.png)
1. Sequential staining of seven markers on the BOND RX.
![](https://hillmanresearch.upmc.edu/wp-content/uploads/phenoimager-ROI-analysis.png)
2. Whole slide scanning with the PhenoImager HT
3. Select regions of interest in Phenochart
4. Analysis of ROIs using inForm/phenoptrReports