The Translational Pathology Imaging Laboratory (TPIL) permits interrogation of protein biomarkers in tissue through next-generation, quantitative histopathologic technologies. Multispectral imaging provides superior image quality through signal amplification and simultaneous removal of tissue autofluorescence. TPIL utilizes Akoya Bioscience’s PhenoImager® platform which includes multiplex IF reagent kits, multispectral imaging on the PhenoImager HT, and image analysis with inForm® software.
Services provided by the Translational Pathology Imaging Laboratory include:
- Panel design and optimization (6 antibodies + DAPI)
- Automated multiplex staining on the Leica BOND RX
- Slide Imaging on the PhenoImager HT
- Whole slide spectral unmixing
- Image analysis with inForm® – includes tissue/cell segmentation algorithms and quantification of individual biomarkers and phenotypes
- Additional custom analyses – biomarker co-expression, spatial relationships between cellular phenotypes
- inForm® workstation availability for independent use
- HALO image analysis software (Indica Labs) availability for independent use.
Multiplex Staining and Imaging Workflow
1. Sequential staining of seven markers on the BOND RX.
2. Whole slide scanning with the PhenoImager HT
3. Select regions of interest in Phenochart
4. Analysis of ROIs using inForm/phenoptrReports
