The mission of the Hillman Cytometry Facility (CF) is to:
- Train investigators, their students and staff to use analytical cytometry to its fullest advantage in their research.
- Basic and advanced training in the use of all facility cytometers is offered continuously.
- The core facility is available to trained users 24 hours a day, seven days a week.
- Provide high quality cell separation by fluorescence activated cell sorting (FACS);
- The CF provides both user-operated and staff-operated cell sorting service. Training for self-operated use of the Sony sorter is available. Staff assisted projects are run on the Thermo Bigfoot duel conventional / spectral high-speed sorter.
- Facility is operated as a BSL-2+ facility and can sort live human cells and cells transfected with retroviral constructs.
- Operate and maintain the cytometers as well as the NanoString platforms.
- The multi-omics assays that the CF performs is a certified (by NanoString) service and as such, aids with project and study design, sample preparation, probe sequencing and facilitating avenues for data analysis.
- Consult on experimental design, analysis and assay development on flow cytometry and multi-omics studies, available to Hillman investigators without charge.
Learn more about our services.
Contact
Tullia Bruno, PhD
Cytometry Facility Director
tbruno@pitt.edu
Armando P. Signore, PhD
Director of Operations
signorea3@upmc.edu
Michael Meyer
Cytometry Facility Manager
meyere@upmc.edu
Shelley Reynolds
Flow Cytometry Technician
reynoldssl4@upmc.edu
Aarti Choudhary, MS
Flow Cytometry Technician
choudharya3@upmc.edu
Location
Primary Location
Hillman Cancer Center Research Pavilion, Lab 1.45
Phone: 412-623-3282
Secondary Location
Assembly, Labs G530,G532, G536
